Application

Sheep Anti-Mouse IgG Antibody, Species absorbed, Biotin conjugate is an antibody against Sheep Mouse IgG for use in Immunohistochemistry.

Research Sub CategoryFragment Specific Secondary Antibodies

Immunohistochemistry Analysis: A representative lot was validated by immunohistochemistry staining (1:500 dilution) of human liver tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Immunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fcg receptors which interact with sialylated Fc fragments on IgG molecules.

Immunogen

Purified mouse IgG.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified sheep polyclonal antibody in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Fractionated by octanoic acid and ammonium sulfate cut, and then depleted with rat IgG column and mouse IgA column prior to biotin conjugation.

Quality

Evaluated by Immunohistochemistry in human prostate tissue.

Immunohistochemistry Analysis: Performance of this biotin-conjugated secondary antibody has been validated by immunohistochemistry staining (1:500 dilution) of human prostate tissue section following a mouse monoclonal anti-CREB primary antibody (Cat. No. MAB5432-C).

Specificity

This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reactivity toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.

Based on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG>>IgG2a>IgG>>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed.